ABSTRACT
Abstract L-Malic acid is the Active Pharmaceutical Ingredient of the latest generation of compound electrolyte injection (STEROFUNDIN ISO, Germany) and plays a very important role in the rescue of critically ill patients. The optical purity of L-malic acid is a Critical Quality Attributes. A new reversed-phase high performance liquid chromatography (RP-HPLC) method for pre-column derivatization of D-malic acid enantiomer impurity in L-malic acid bulk drug was established. The derivatization reaction was carried out using (R)-1-(1-naphthyl)ethylamine ((R)-NEA) as a chiral derivatization reagent. The Kromasil C18 column was used with a detection wavelength of 225 nm, a flow rate of 1.0 mL·min-1, and a column temperature of 30 °C. The mobile phase was acetonitrile-0.01 mol·L-1 potassium dihydrogen phosphate solution (containing 20 mmol·L-1 sodium heptanesulfonate, adjusted to pH 2.80 with phosphoric acid) (at a ratio of 45:55) and the resolution of D-malic acid and L-malic acid derivatization products reached 1.7. The proposed method possesses the advantages of simple operation, mild conditions, stable derivatization products and low cost. Also it gave better separation and was more accurate than previous methods
Subject(s)
Chromatography, High Pressure Liquid/methods , Malicum Acidum/analysis , Chromatography, Reverse-Phase/methods , Patients/classification , Total Quality Management/classificationABSTRACT
Este trabalho consiste em uma pesquisa realizada sobre a utilização de líquidos irrigantes por cirurgiões dentistas na cidade de Itabuna-BA. Para isto, delimitou-se a seguinte problemática: quais são as escolhas dos líquidos irrigantes nos tratamentos endodônticos com o intuito de promover uma total desinfecção dos canais radiculares? E como objetivo geral, elucidar sobre as escolhas dos cirurgiões dentistas localizados na cidade de Itabuna-Bahia em relação as substâncias utilizadas como coadjuvantes no tratamento endodôntico durante a etapa de irrigação. A pesquisa teve uma abordagem qualitativa e foi utilizado o instrumento questionário com cem dentistas da cidade selecionada. Este questionário consistia em oito perguntas, dentre elas qual irrigante utilizado e a porcentagem do mesmo. Com relação aos resultados, pode-se afirmar que no tratamento endodôntico, o hipoclorito de sódio continua sendo a solução irrigante de escolha entre os profissionais, diferentes concentrações de soluções de hipoclorito de sódio são empregadas durante o preparo biomecânico, por endodontistas e clínicas gerais que praticam a endodontia, não existindo uma unanimidade quanto a escolha, nenhum acordo existe em relação a sua concentração ideal(AU)
This work consists of a research carried out on the use of irrigating liquids by dental surgeons in the city of Itabuna-BA. For this, the following problem was defined: what are the choices of irrigating liquids in endodontic treatments in order to promote a total disinfection of the root canals? And as a general objective, elucidate about the choices of dentists located in the city of Itabuna-Bahia in relation to the substances used as adjuvants in endodontic treatment during the irrigation stage. The research had a qualitative approach and the questionnaire was used with 100 dentists in the selected city. This questionnaire consisted of eight questions, including which irrigant was used and the percentage of it. Regarding the results, it can be said that in endodontic treatment, sodium hypochlorite remains the irrigating solution of choice among professionals, different concentrations of sodium hypochlorite solutions are used during biomechanical preparation by endodontists and general practitioners who practice endodontics, there is no unanimity as to the choice, no agreement exists regarding their ideal concentration(AU)